A well-designed and -optimized polymerase chain reaction (PCR)-based assay has the potential to be a highly sensitive and highly specific nucleic acid target detection method, relative to other diagnostic methods. However, even good PCR assays have limitations as there are many factors which have to be controlled to ensure the success of a PCR run. continue reading : TROUBLESHOOTING GUIDE FOR REAL-TIME PCR RUNS FOR SARS-CoV-2 DETECTION