Techniques and experiments in molecular biology employed in the study of infectious diseases greatly rely on information obtained by studying the genetic material from pathogens or of infected individuals. It is important that the laboratory work areas are clean and free from nucleases that can degrade DNA or RNA. Aside from work areas, commonly used continue reading : PROCEDURE FOR DMPC TREATMENT OF GLASSWARE AND PLASTICWARE
A well-designed and -optimized polymerase chain reaction (PCR)-based assay has the potential to be a highly sensitive and highly specific nucleic acid target detection method, relative to other diagnostic methods. However, even good PCR assays have limitations as there are many factors which have to be controlled to ensure the success of a PCR run. continue reading : TROUBLESHOOTING GUIDE FOR REAL-TIME PCR RUNS FOR SARS-CoV-2 DETECTION